Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish...
Wildlife research has been indispensable for increasing our insight into ecosystem functioning as well as for designing effective conservation measures under the currently high rates of biodiversity loss. Genetic and genomic analyses might be able...
Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and...
Routine sample collection for genotyping genetically modified animals (rodents) (GMAs) typically includes tail snip, toe clip, or ear biopsy. To decrease the potential pain and distress caused by tissue sample collection for genetic analysis, we...
A survey was conducted regarding zebrafish Danio rerio use for scientific research with a focus on: anaesthesia and euthanasia; housing and husbandry; breeding and production; refinement opportunities. A total of 98 survey responses were received...
In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified...
Fin clipping of live fish under anaesthesia is widely used to collect tissues samples for DNA extraction. However, this technique raises a number of ethical concerns, since the use of anaesthetic and/or physical fin damage...
The zebrafish has become a valuable research model within the laboratory. Currently the proven method of genotyping involves taking a tail fin clip from the fish to gain the sample. This involves surgically removing a...
Various animal models are indispensible in biomedical research. Increasing awareness and regulations have prompted the adaptation of more humane approaches in the use of laboratory animals. With the development of easier and faster methodologies to...
Genotyping of genetically-engineered mice is necessary for the effective design of breeding strategies and identification of mutant mice. This process relies on the identification of DNA markers introduced into genomic sequences of mice, a task...