Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

The zebrafish (Danio rerio) is widely used as a promising translational model organism for studying various brain disorders. Zebrafish are also commonly used in behavioral and drug screening assays utilizing individually tested (socially isolated) fish...

Zebrafish has become an animal model in research and articles have established ideal conditions for their maintenance. However, little is known regarding the influence of gender and other cues on zebrafish behavior. Thus, here we...

Despite the increasing popularity of zebrafish (Danio rerio) as an animal model, the environmental enrichment preferences of this species have been largely unexplored. We sought to determine the preferences of mature female zebrafish that were...

Environmental enrichment is used extensively to improve the welfare of animals in captivity, however, the importance of physical exercise as enrichment is only now coming to light. A key question in assessing the welfare of...

Environmental enrichment, or the enhancement of an animal’s surroundings when in captivity to maximise its wellbeing, has been increasingly applied to fish species, particularly those used commercially. Laboratory species could also benefit from enrichment, but...

This open access book is a printed edition of the Special Issue on Welfare of Cultured and Experimental Fishes that was published in the journal Fishes. The PDF is free for download.

We compared preferences shown by zebrafish (Danio rerio) and three-spined stickleback (Gasterosteus aculeatus) for shelter provided by above-tank shade and artificial plants. Zebrafish showed no preference for either shelter, whereas sticklebacks showed a preference for...

Environmental enrichment involves increasing the complexity of a fish's environment in order to improve welfare. Researchers are legally obliged to consider the welfare of laboratory animals and poor welfare may result in less robust data...

This study examined the effects of simple changes in the tank environment on the wellbeing of laboratory-maintained zebrafish Danio rerio. Groups of D. rerio were either housed in stable environments (where they were maintained in...

Environmental stimuli are critical in preclinical research that utilizes laboratory animals to model human brain disorders. The main goal of environmental enrichment (EE) is to provide laboratory animals with better choice of activity and greater...

Zebrafish are now one of the most used animal model species in scientific research worldwide. Our current knowledge of wild zebrafish is limited to an estimated range of their natural habitats and their tendencies to...

Cognitive stimulation has been shown to be rewarding and capable of eliciting positive emotions in several species. In contrast to the abundant learning and exploration opportunities available in nature, captive environments can be under-stimulating—with the...

A survey was conducted regarding zebrafish Danio rerio use for scientific research with a focus on: anaesthesia and euthanasia; housing and husbandry; breeding and production; refinement opportunities. A total of 98 survey responses were received...

Recently environmental enrichment for laboratory housed fish has received increasing levels of attention from a variety of interested parties from hobbyists, through animal technologists and aquaculturists, to scientists. Over the last few years, the growth...

Environmental enrichment provides laboratory-housed species the opportunity to express natural behavior and exert control over their home environment, thereby minimizing stress. We sought to determine whether providing an artificial plant in the holding tank as...

The inhibitory avoidance paradigm allows the study of mechanisms underlying learning and memory formation in zebrafish (Danio rerio Hamilton). For zebrafish, the physiology and behavior associated with this paradigm are as yet poorly understood. We...

Sexually mature zebrafish were housed as single male-female pairs with or without plastic vegetation for 1, 5, or 10 d for comparison of whole-body cortisol measured by radioimmunoassay. Individually housed male zebrafish were used as...

This article discusses how behavioral management, enrichment structures, diet, and light cycle can be used to help improve the welfare of zebrafish.

Although environmental enrichment is known to improve laboratory rodent wellbeing and enhance scientific data collection, relatively little is known with regards to the type of enrichment that might be useful for zebrafish (Danio rerio). Therefore...

This is the third volume of discussions that took place on the Laboratory Animal Refinement & Enrichment Forum (LAREF). This forum is dedicated to the exchange of personal experiences of refining the conditions under which...

Enrichment of the environment for captive animals is aimed at producing beneficial effects on the behaviour and physiology of relevant species, and is commonly used to reduce harmful social behaviours and stereotypies. However, little work...

The zebrafish (Danio rerio) is a small tropical minnow that is playing an increasingly big role in the world of scientific research. In the last year alone, researchers working with this diminutive fish from south...

Information about the welfare and husbandry of pet and laboratory fish is scarce although millions of fish are sold in pet shops and used in laboratory research every year. Inadequate housing conditions can cause behavioural...

Comparative studies on neural plasticity in non-mammalian vertebrates are increasingly promoted as an important complement to mammalian models. In teleost fishes the number of brain cells increases with age, body weight, and body length throughout...

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