Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
McMahon, S. A., Wigham, E. 2020. ‘All Ears’: A questionnaire of 1516 owner perceptions of the mental abilities of pet rabbits, subsequent resource provision, and the effect on welfare. Animals 10(10), 1730.

Pet rabbit welfare is a hidden crisis: Inappropriately housed, fed, and not routinely provided healthcare—rabbits can often suffer painful conditions and shortened lifespans. This study provides novel understanding of owners’ perceptions of rabbits’ mental capabilities...

Rault, J.-L., Waiblinger, S., Boivin, X. et al. 2020. The power of a positive human–animal relationship for animal welfare. Frontiers in Veterinary Science 7, 590867.

Domestic animals often seek and enjoy interacting with humans. Positive human–animal relationships can elicit positive emotions and other positive welfare outcomes. Nevertheless, our understanding of the underlying processes that govern the positive perception of humans...

Rojas, D., Broom, D. M., Orihuela, A. et al. 2020. Effects of human-animal relationship on animal productivity and welfare. Journal of Animal Behaviour and Biometeorology 8(3), 196-205.

This is a literature review of the effects of humans' relationships with farm animals on animal productivity and welfare, including the following topics: definition of the concept and description of different tests that have been...

Heintz, M. R.; Fuller, G., Allard, S. 2019. Exploratory investigation of infrared thermography for measuring gorilla emotional responses to interactions with familiar humans. Animals 9(9), 604.

Interactions between zoo professionals and animals occur regularly and are believed to be enriching for animals. Little empirical information exists on how animals perceive these interactions, and particularly how the interactions affect the emotional states...

McMurray, C., Allen, N., Hook, S. et al. 2018. I3S software as a refinement technique to identify individuals within a captive Scyliorhinus canula population. Animal Technology and Welfare 17(3), 204-205 (IAT Congress 2018 Poster Presentation).

Demarking individual animals within a group is often required in research. However, the process of invasive tagging induces stress and if a tag becomes detached it may wound the animal and/or prevent identification. Photo identification...

Lee, L. Y., Lee, D., Ryu, H. et al. 2019. Capnography-guided endotracheal intubation as an alternative to existing intubation methods in rabbits. JAALAS 58(2), 240-245.

Rabbits provide a unique challenge for routine endotracheal intubation in clinical practice because of various distinctive anatomic and physiologic features. Many previously proposed methods for endotracheal intubation in rabbits are limited by several factors, including...

Message, R., Greenhough, B. 2019. “But it’s just a fish”: Understanding the challenges of applying the 3Rs in laboratory aquariums in the UK. Animals 9(12), 1075.

Adopting a social science perspective and qualitative methodology on the problem of laboratory fish welfare, this paper examines some underlying social factors and drivers that influence thinking, priorities and implementation of fish welfare initiatives and...

Mather, J. A. 2019. Ethics and care: For animals, not just mammals. Animals 9(12), 1018.

Animals come in a huge array of types, species and structures, but without realizing it, we have focused our care on those like us, mammals. They look something like us, so it's easy to empathize...

González-Pech, P. G., Marín-Tun, C. G., Valladares-González, D.A. et al. 2018. A protocol of human animal interaction to habituate young sheep and goats for behavioural studies. Behavioural Processes 157, 632-637.

Animal habituation is key to obtain reliable data on behavioural studies but detailed procedures to achieve it are scarce. This study designed a set of actions to habituate sheep and goats to human observers. Pelibuey...

Nawroth C., Albuquerque N., Savalli C. et al. 2018. Goats prefer positive human emotional facial expressions. Royal Society Open Science 5(8), 180491.

Domestication has shaped the physiology and the behaviour of animals to better adapt to human environments. Therefore, human facial expressions may be highly informative for animals domesticated for working closely with people, such as dogs...

Ruberto, T., Clément, R. J. G., Spinello, C. et al. 2018. The tagging procedure of visible implant elastomers influences zebrafish individual and social behavior. Zebrafish 15(5), 433-444.

One commonly used method to preserve individual identity in the study of social behavior of zebrafish is through silicone-based visible implant elastomers (VIEs), which represent a safe and durable tagging procedure. While the effects of...

Gut, W., Crump, L., Zinsstag, J. et al. 2018. The effect of human interaction on guinea pig behavior in animal-assisted therapy. Journal of Veterinary Behavior 25, 56-64.

Guinea pigs are included in various animal-assisted interventions (AAIs), but no research has been published to date on behavioral changes in guinea pigs interacting with humans. The goal of this study was to evaluate the...

Cronin, K. A., Bethell, E. J., Jacobson, S. L. et al. 2018. Evaluating mood changes in response to anthropogenic noise with a response-slowing task in three species of zoo-housed primates. Animal Behavior and Cognition 5(2), 209–221.

In the zoo environment, anthropogenic noise is common as sound levels fluctuate due to visitors, construction, habitat design, and special events. In this study, changes in the mood of three species of zoo-housed primates in...

Miranda, A., Pego, J. M., Correia-Pinto, J. 2017. Animal facility videoendoscopic intubation station: Tips and tricks from mice to rabbits. Laboratory Animals 51(2), 204-207.

Endotracheal intubation of laboratory animals is a common procedure shared by several research fields for different purposes, such as mechanical ventilation of anaesthetized animals, instillation of cytotoxic nanoparticles, infectious agents or tumour cells for induction...

de Lima Rocha, A. D., Menescal-de-Oliveira, L., da Silva, L. F. S. 2017. Effects of human contact and intra-specific social learning on tonic immobility in guinea pigs, Cavia porcellus. Applied Animal Behaviour Science 191, 1-4.

Social learning is the capacity of animals to acquire adaptive information from others. In the case of fear responses, animals can learn fearful or non-fearful responses by observing the behavior of conspecifics. Tonic immobility (TI)...

O'Connell, C. P., Leurs, G. 2016. A minimally invasive technique to assess several life-history characteristics of the endangered great hammerhead shark Sphyrna mokarran. Journal of Fish Biology 88(3), 1257-1264.

A dorsal-fin photo-identification technique paired with a non-invasive parallel laser photogrammetry technique was used to non-invasively identify individual Sphyrna mokarran over time. Based on the data collected over a duration of 59 days, 16 different...

Bayne, K. A., Beaver, B. V., Mench, J. A. et al. 2015. Laboratory animal behavior. In: Laboratory Animal Medicine, 3rd edition. Fox, J. G., Anderson, L., Otto, G., et al. (eds). Elsevier, Inc., Amsterdam, NL. pp. 1617-1651.

The study of laboratory animal behavior has increased steadily over the last decade, with expanding emphasis on a variety of commonly used species. In the United States, this trend was initially focused on species for...

Nicholson, G., Sheldon, E. L., Hamilton, J. et al. 2014. Sand pools as a novel enrichment for rabbits. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 571. (Abstract #P83).

For anyone who has housed rabbits in an artificial plastic environment, what to provide as a nonfood enrichment can be an issue. Nonfood enrichment, or environmental enrichment, can be any toy, engaging sights or sounds...

Chelluri, G. I., Ross, S. R., Wagner, K. E. 2013. Behavioral correlates and welfare implications of informal interactions between caretakers and zoo-housed chimpanzees and gorillas. Applied Animal Behaviour Science 147, 306-315.

In captive animal facilities, human staff members are a relevant part of the animals’ social environment in addition to providing care and managing the social group. Structured, predictable interactions and relaxed, spontaneous contacts may all...

Reinhardt, V. (ed.) 2013. Compassion Makes a Difference - Discussions by the Laboratory Animal Refinement & Enrichment Forum [LAREF], Volume III. Animal Welfare Institute, Washington, DC.

This is the third volume of discussions that took place on the Laboratory Animal Refinement & Enrichment Forum (LAREF). This forum is dedicated to the exchange of personal experiences of refining the conditions under which...

Blyth, L. 2012. A safe, gentle and effective method of oral dosing guinea-pigs. Animal Technology and Welfare 11, 187-188.

Guinea pigs are difficult to oral gavage dose. Previous adverse effects from this dosing method, using rigid catheters, had caused an earlier endpoint to studies and loss of data. We therefore developed a prototype gag...

Swennes, A. G., Alworth, L. C., Harvey, S. B. et al. 2011. Human handling promotes compliant behavior in adult laboratory rabbits. JAALAS 50(2), 41-45.

Routine laboratory procedures can be stressful for laboratory animals. We wanted to determine whether human handling of adult rabbits could induce a degree of habituation, reducing stress and facilitating research-related manipulation. To this end, adult...

Hawkins, P., Burn, C., Hurst, J. et al. 2011. Report of the 2010 RSPCA/UFAW rodent welfare group meeting - The effect of husbandry on welfare and promoting good practice. Animal Technology and Welfare 10(2), 105-114.

The RSPCA/UFAW Rodent Welfare Group holds a one-day meeting every autumn so that its members can discuss current welfare research, exchange views on rodent welfare issues and share experiences of the implementation of the 3Rs...

Coleman, K. 2011. Caring for nonhuman primates in biomedical research facilities: Scientific, moral and emotional considerations. American Journal of Primatology 73(3), 220-225.

Animal care for nonhuman primates (NHPs) in biomedical facilities has undergone major changes in the past few decades. Today, most primate facilities have dedicated and highly trained animal care technicians who go to great efforts...

Moreau, E. 2010. To squeeze or not to squeeze? A Discussion on LAREF, September, 2009. Laboratory Primate Newsletter 49(2), 3-4.

The discussion was started by the following questions: "Is the squeeze-back mechanism more or less stressful than the pole and collar system for removing a macaque from his cage for an IM (intramuscular) injection?" and...