Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
Harvey, A. M., Beausoleil, N. J., Ramp, D. et al. 2023. Mental experiences in wild animals: Scientifically validating measureable welfare indicators in free-roaming horses. Animals 13(9), 1507.

The mental experiences of animals are what characterises their welfare status. The Five Domains Model for assessing welfare aligns with the understanding that physical and mental states are linked. Following measurement of indicators within each...

Marks, C. A., Allen, L., Lindeberg, H. 2023. Non-lethal dose-response models replace lethal bioassays for predicting the hazard of para-animopropiophenone to Australian wildlife. Animals 13(3), 472.

Para-aminopropiophenone (PAPP) is a potent methaemoglobin (MetHb) forming agent used for the lethal control of exotic carnivores and mustelids. To assess the sensitivity of Australian wildlife to PAPP we developed an in vivo assay that...

Padalino, B., Benedetti, B., Felici, M. et al. 2023. GLAD scale for ranking welfare of horses on arrival after transport to slaughterhouses. Animals 13(9), 1465.

To date, there is no official method for measuring horse welfare after transport. This study aimed to develop a scale to classify horses into four categories: good shape; light affected; affected; down (GLAD) based on...

Parsons, M., Stryjek, R., Fendt, M. et al. 2023. Making a case for the free exploratory paradigm: Animal welfare-friendly assays that enhance heterozygosity and ecological validity. Frontiers in Behavioral Neuroscience 17, 1228478.

Animal welfare considerations are an essential part of scientific research. If animals may suffer, then even 26 the most promising research plans can be delayed or rejected and the resulting data may be questionable. 27...

Platto, S., Serres, A. 2023. A quantitative assessment of trainers-dolphins’ interactions in the absence of food reward. Animals 13(10), 1651.

All the studies that have considered the motivation of the dolphins to interact with their trainers as a possible welfare indicator have been carried out in facilities where the trainer-dolphin interactions (TDIs) sessions were reinforced...

Seganfreddo, S., Fornasiero, D., De Santis, M. et al. 2023. A pilot study on behavioural and physiological indicators of emotions in donkeys. Animals 13(9), 1466.

Recognizing animal emotions is critical to their welfare and can lead to a better relationship with humans and the environment, especially in a widespread species like the donkey, which is often prone to welfare issues...

Warwick, C., Pilny, A., Steedman, C. et al. 2023. Mobile zoos and other itinerant animal handling events: Current status and recommendations for future policies. Animals 13(2), 214.

Mobile zoos are events in which non-domesticated (exotic) and domesticated species are transported to venues such as schools, hospitals, parties, and community centres, for the purposes of education, entertainment, or social and therapeutic assistance. We...

Williams, E., Hunton, V., Hosey, G. et al. 2023. The impact of visitors on non-primate species in zoos: A quantitative review. Animals 13(7), 1178.

Visitors are a prominent feature in the lives of zoo animals, and their presence can cause a range of impacts on zoo animals (typically classed as positive, negative or neutral impacts), commonly referred to as...

Buenhombre, J., Daza-Cardona, E. A., Sousa, P. et al. 2023. Structural environmental enrichment and the way it is offered influence cognitive judgement bias and anxiety-like behaviours in zebrafish. Animal Cognition 26, 563–577.

Environmental enrichment in zebrafish generally reduces anxiety-related behaviours, improves learning in maze trials and increases health and biological fitness. However, certain types of enrichment or certain conditions induce the opposite effects. Therefore, it is essential...

Lupfer, G., Brandenburger, A., Machado, M. et al. 2023. Ultrasonic vocalizations near 30 kHz may indicate excitement rather than distress in female Wistar rats. Applied Animal Behaviour Science 261, 105881.

Rats emit ultrasonic vocalizations (USVs), sometimes referred to as 50-kHz vocalizations, during activities such as play and lower-frequency USVs, sometimes referred to as 22-kHz vocalizations, when experiencing distress. Definitions of 22-kHz vocalizations vary in terms...

Ricci-Bonot, C., Mills, D. S. 2023. Recognising the facial expression of frustration in the horse during feeding period. Applied Animal Behaviour Science 265, 105966.

Horses often present negative emotional states which are frequently poorly recognised, with much of our understanding of horse expressions based on anecdotes, rather than scientific evidence. The aim of this project was to identify potential...

Fontana, B. D., Alnassar, N., Parker, M. O. 2023. Transgenerational effects of early-life stress on anxiety behavior in zebrafish (Danio rerio). Behavioural Processes 208, 104874.

Early-life adversity impacts on anxiety-related behaviors in adulthood. The effects of such adversity not only affect the animal itself, but can be passed on transgenerationally. Pervasive effects of experimentally-induced early-life stress (ELS) have been documented...

Domínguez-Oliva, A., Hernández-Ávalos, I., Olmos-Hernández, A. et al. 2023. Thermal response of laboratory rats (Rattus norvegicus) during the application of six methods of euthanasia assessed by infrared thermography. Animals 13(18), 2820.

Refinement is one of the principles aiming to promote welfare in research animals. The techniques used during an experimental protocol, including euthanasia selection, must prevent and minimize suffering. Although the current euthanasia methods applied to...

Domínguez-Oliva, A., Olmos-Hernández, A., Hernández-Ávalos, I. et al. 2023. Rat grimace scale as a method to evaluate animal welfare, nociception, and quality of the euthanasia method of wistar rats. Animals 13(20), 3161.

Refinement of experimental procedures in animal research has the objective of preventing and minimizing pain/distress in animals, including the euthanasia period. This study aimed to evaluate pain associated with six methods of euthanasia in Wistar...

Dybdal, N., Horgan, M., Costa, L. et al. 2023. Equine gunshot euthanasia: Creation of a 3D-printed model with integrated sensors for training. Animals 13(16), 2566.

Challenges and issues related to the use of pentobarbital euthanasia and disposal of animal remains within the US have recently been reviewed. Environmental and public health challenges increasingly necessitate consideration of alternative methods such as...

Fletcher, K. A., Limon, G., Padalino, B. et al. 2023. Impact of social buffering and restraint on welfare indicators during uk commercial horse slaughter. Animals 13(14), 2276.

Current legislation in the United Kingdom stipulates that horses should not be slaughtered within sight of one another. However, abattoir personnel anecdotally report that, for semi-feral horses unused to restraint, co-slaughtering alongside a conspecific could...

Stevens, C., Hammonds, T., Hinchcliffe, J. et al. 2023. Report of the 2022 RSPCA/UFAW Rodent Welfare Group Meeting. Animal Technology and Welfare 22(2), 91–100.

The RSPCA/UFAW Rodent Welfare Group has held a one-day meeting every autumn for the last 29 years, so that its members can discuss current welfare research, exchange views on welfare issues and share experiences of...

Xue, B., Ma, Y.-Y., Zhu, J.-Y. et al. 2023. Chronic social comparison elicits depression- and anxiety-like behaviors and alterations in brain-derived neurotrophic factor expression in male rats. Animal Cognition 26(5), 1505–1519.

Social comparison is a fundamental human characteristic; however, long-term social comparison may induce psychological stress and can lead to depression and anxiety. Recent studies have shown that nonhuman primates compare themselves with others; however, no...

Acharya, R. Y., Hemsworth, P. H., Coleman, G. J. et al. 2022. The animal-human interface in farm animal production: Animal fear, stress, reproduction and welfare. Animals 12(4), 487.

A negative human-animal relationship (HAR) from the perspective of the animal is a limiting factor affecting farm animal welfare, as well as farm animal productivity. Research in farm animals has elucidated sequential relationships between stockperson...

Hickman, D. L. 2022. Evaluation of carbon dioxide euthanasia of female Sprague Dawley rats alone or with unfamiliar conspecifics. JAALAS 61(2), 195-200.

Most studies evaluating methods of euthanasia to date have focused on the euthanasia of individual animals. However, larger chambers are commonly used to euthanize multiple cages of animals at once. This study evaluated the use...

Jardat, P., Calandreau, L., Ferreira, V. et al. 2022. Pet-directed speech improves horses' attention toward humans. Scientific Reports 12(1), 4297.

In a recent experiment, we showed that horses are sensitive to pet-directed speech (PDS), a kind of speech used to talk to companion animals that is characterized by high pitch and wide pitch variations. When...

Davis, A. K., Garner, J. P., Chu, D. K. et al. 2022. Propofol immersion as a euthanasia method for adult zebrafish (Danio rerio). Comparative Medicine 72(3), 204-209.

The exponential rise of the zebrafish (Danio rerio) as a model organism in biomedical research has far outstripped our un- derstanding of basic husbandry and welfare for this species. As a case in point, here...

Hickman, D. L. 2022. Minimal exposure times for irreversible euthanasia with carbon dioxide in mice and rats. JAALAS 61(3), 283-286.

When using an anesthetic overdose to euthanize laboratory rodents, a secondary method of euthanasia is recommended to ensure that the apparent death is irreversible. This secondary method usually is accomplished through the collection of tissues...

Zausinger, P., Schneider, B. M., Bauer, A. et al. 2022. Short communication: Do the tameness of the dams and the sex of the offspring have an influence on the “gentling” effect in laboratory rats? Journal of Veterinary Behavior 52-53, 45-49.

“Gentling” (soft touching, “taming”) has long-term effects on the behavior of laboratory rats (Rattus norvegicus). Our own studies showed that the use of a “gentling” program in the fourth and fifth week of life resulted...

Clarkson, J. M., Martin, J. E., McKeegan, D. E. F. 2022. A review of methods used to kill laboratory rodents: Issues and opportunities. Laboratory Animals 56(5), 419-436.

Rodents are the most widely used species for scientific purposes. A critical pre-requisite of their use, based on utilitarian ethical reasoning, is the provision of a humane death when necessary for scientific or welfare grounds...