Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
Kitchenham, L., MacLellan, A., Paletta, P. et al. 2024. Do housing-induced changes in brain activity cause stereotypic behaviours in laboratory mice? Behavioural Brain Research 462, 114862.

Abnormal repetitive stereotypic behaviours (SBs) (e.g. pacing, body-rocking) are common in animals with poor welfare (e.g. socially isolated/in barren housing). But how (or even whether) poor housing alters animals’ brains to induce SBs remains uncertain...

Silva-Almeida, C., Muniz, S. C. A., Jobim, C. M. N. et al. 2024. Perinatal environmental enrichment changes anxiety-like behaviours in mice and produces similar intergenerational benefits in offspring. Behavioural Brain Research 456, 114700.

Environmental enrichment implemented in early life is able to induce long-term changes in gene expression, synaptic function and behavioural responses. In this study, we evaluated the adult behavioural effects of perinatal environment enrichment in male...

Robinson, L. M., Weiss, A. (Eds.) 2023. Nonhuman Primate Welfare: From History, Science, and Ethics to Practice. Springer, Cham, Switzerland. 671 p.

This volume reviews the broad topic of welfare in nonhuman primates under human care. Chapters detail the history of primates in captivity, ethical and legal issues surrounding the use of nonhuman primates as entertainment or...

Ausderau, K. K., Colman, R. J., Kabakov, S. et al. 2023. Evaluating depression- and anxiety-like behaviors in non-human primates. Frontiers in Behavioral Neuroscience 16, 1006065.

Depression and anxiety are some of the most prevalent and debilitating mental health conditions in humans. They can present on their own or as co-morbidities with other disorders. Like humans, non-human primates (NHPs) can develop...

D’Alessio, R. M., Hanlon, A., O’Driscoll, K. 2023. Comparison of single- and double-spaced feeders with regard to damaging behavior in pigs. Frontiers in Veterinary Science 10, 1073401.

This work compared the behavior and performance of 24 groups of 12 pigs kept in pens with either a DOUBLE [one feed space aligned with the front wall of the pen (WALL), and one immediately...

Parsons, M., Stryjek, R., Fendt, M. et al. 2023. Making a case for the free exploratory paradigm: Animal welfare-friendly assays that enhance heterozygosity and ecological validity. Frontiers in Behavioral Neuroscience 17, 1228478.

Animal welfare considerations are an essential part of scientific research. If animals may suffer, then even 26 the most promising research plans can be delayed or rejected and the resulting data may be questionable. 27...

Watanabe, S. 2023. Infrared thermography for non-invasive measurement of social inequality aversion in rodents and potential usefulness for future animal-friendly studies. Frontiers in Behavioral Neuroscience 17, 1131427.

Infrared thermography is a method that detects thermal radiation energy and can measure the body surface temperature of animals from a distance. While rectal temperature has traditionally been used to measure animals’ core temperature, thermal...

Verspeek, J., Stevens, J. M. G. 2023. Behavioral and physiological response to inequity in bonobos (Pan paniscus). American Journal of Primatology 85(1), e23455.

Inequity aversion (IA), the affective, cognitive, and behavioral response to inequitable outcomes, allows individuals to avoid exploitation and therefore stabilizes cooperation. The presence of IA varies across animal species, which has stimulated research to investigate...

Lupfer, G., Brandenburger, A., Machado, M. et al. 2023. Ultrasonic vocalizations near 30 kHz may indicate excitement rather than distress in female Wistar rats. Applied Animal Behaviour Science 261, 105881.

Rats emit ultrasonic vocalizations (USVs), sometimes referred to as 50-kHz vocalizations, during activities such as play and lower-frequency USVs, sometimes referred to as 22-kHz vocalizations, when experiencing distress. Definitions of 22-kHz vocalizations vary in terms...

Alejandro, J., Yamanashi, Y., Nemoto, K. et al. 2023. Reducing stress and stereotypic behaviors in captive female pygmy slow lorises (Nycticebus pygmeaus). American Journal of Primatology 85(7), e23495.

Improving captive conditions of pygmy slow lorises (Nekaris and Nijman have recently suggested that the pygmy slow loris should be called the pygmy loris and is distinctive enough to warrant a new genus, Xanthonycticebu) (Nycticebus...

Luisi, B., Micheletta, J., Julle-Danière, E. et al. 2023. Monkeying around: Non-human primate behavioural responses to humans reproducing their facial expressions. Applied Animal Behaviour Science 265, 105990.

People are often observed mimicking animals’ facial expressions in an attempt to communicate with them. However, to date, there is limited understanding of how animals respond to humans reproducing their facial displays, or if this...

Moëne, O. L., Larsson, M. 2023. A new tool for quantifying mouse facial expressions. ENeuro 10(2) ENEURO.0349-22.2022.

Facial expressions are an increasingly used tool to assess emotional experience and affective state during experimental procedures in animal models. Previous studies have successfully related specific facial features with different positive and negative valence situations...

Clark, F. E. 2023. In the zone: Towards a comparative study of flow state in primates. Animal Behavior and Cognition 10(1), 62–88.

Flow is an altered state of feeling ‘in the zone’ when fully absorbed in a challenge and is associated with positive affective state (feelings). Despite almost five decades of research, Flow has not yet been...

Shanazz, K., Nalloor, R., Vazdarjanova, A. 2023. A mild stressor induces short-term anxiety and long-term phenotypic changes in trauma-related behavior in female rats. Frontiers in Behavioral Neuroscience 17.

IntroductionAnxiety and anxiety-influenced disorders are sexually dimorphic with women being disproportionately affected compared to men. Given the increased prevalence in women and the documented differences in anxiety and trauma behavior between male and female rats...

Stevens, C., Hammonds, T., Hinchcliffe, J. et al. 2023. Report of the 2022 RSPCA/UFAW Rodent Welfare Group Meeting. Animal Technology and Welfare 22(2), 91–100.

The RSPCA/UFAW Rodent Welfare Group has held a one-day meeting every autumn for the last 29 years, so that its members can discuss current welfare research, exchange views on welfare issues and share experiences of...

Tanaka, Y., Nakata, T., Hibino, H. et al. 2023. Classification of multiple emotional states from facial expressions in head-fixed mice using a deep learning-based image analysis. PLOS ONE 18(7), e0288930.

Facial expressions are widely recognized as universal indicators of underlying internal states in most species of animals, thereby presenting as a non-invasive measure for assessing physical and mental conditions. Despite the advancement of artificial intelligence-assisted...

Xue, B., Ma, Y.-Y., Zhu, J.-Y. et al. 2023. Chronic social comparison elicits depression- and anxiety-like behaviors and alterations in brain-derived neurotrophic factor expression in male rats. Animal Cognition 26(5), 1505–1519.

Social comparison is a fundamental human characteristic; however, long-term social comparison may induce psychological stress and can lead to depression and anxiety. Recent studies have shown that nonhuman primates compare themselves with others; however, no...

Zhang, Y., Yu, J., Zhang, Y. et al. 2023. Emotional contagion and social support in pigs with the negative stimulus. Animals 13(20), 3160.

This study expects to confirm the existence of emotional transmission in pigs from multiple perspectives and to provide theoretical references for improving animal welfare in livestock farming. A group that could directly observe (DO) and...

Sharma, S., Khanal, L., Shrestha, S. et al. 2023. Zoo visitors as a source of enrichment to reduce abnormal behavior in captive rhesus macaques (Macaca mulatta) in the Central Zoo, Kathmandu, Nepal. Journal of Animal Behaviour Biometeorology 11(1), e2023005.

Overexpression of abnormal behavior among captive primates indicates poor management practices. The type, frequency, and contributing factors of abnormal behaviors vary highly across individual animals in captive settings. This study explored if sex, rearing history...

Clancy, B. M., Theriault, B. R., Turcios, R. et al. 2023. The effect of noise, vibration, and light disturbances from daily health checks on breeding performance, nest building, and corticosterone in mice. JAALAS 62(4), 291–302.

At our institution, the techniques that technicians use for health checks vary for mice housed in cages on individually ventilated caging (IVC) racks. If the mice cannot be adequately visualized, some technicians partially undock the...

Goldsborough, Z., Sterck, E. H. M., de Waal, F. B. M. et al. 2022. Individual variation in chimpanzee (Pan troglodytes) repertoires of abnormal behaviour. Animal Welfare 31(1), 125-135.

Abnormal behaviour in captive animals is both pervasive and ambiguous. Although individual differences are central to the field of animal welfare, studies on abnormal behaviour predominantly employ quantitative, population-level approaches. For example, whereas previous studies...

Lambert, H., Elwin, A., D’Cruze, N. 2022. Frog in the well: A review of the scientific literature for evidence of amphibian sentience. Applied Animal Behaviour Science 247, 105559.

Millions of amphibians are traded annually around the world for the exotic pet industry. Their experience during both trade, and in captivity as pets, leads to numerous animal welfare issues. The poor welfare of many...

Habenicht, L. M., Staley, A. W., Clancy, B. M. et al. 2022. Characterization of a jumping stereotypy in gerbils (Meriones unguiculatus) and assessment of opaque tubing enrichment on stereotypies and breeding. JAALAS 61(2), 149-158.

Mongolian gerbils can develop stereotypic behaviors, including corner digging. At our institution, gerbils also engage in repetitive corner jumping, which we sought to characterize as a potentially novel stereotypy in gerbils. We then attempted to...

Pan, L., Nian, H., Zhang, R. et al. 2022. Stereotypic behaviors are associated with physiology and immunity differences in long-term confined sows. Physiology & Behavior 249, 113776.

Pregnant sows in the confined environment have poor welfare and frequently perform stereotypic behaviors. In order to clarify whether highly stereotypic behavior is a sign of increased stress and successfully contributes to coping with or...

Zapata Cardona, J., Ceballos, M. C., Tarazona Morales, A. M. et al. 2022. Music modulates emotional responses in growing pigs. Scientific Reports 12(1), 3382.

There is a lack of clarity on whether pigs can emotionally respond to musical stimulation and whether that response is related to music structure. Qualitative Behavioral Assessment (QBA) was used to evaluate effects of 16...