Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
Scotto, J. 2012. Group housing male rabbits: Yes, it can be done. Tech Talk [The Newsletter for Laboratory Animal Science Technicians] 17(6), 3.

Since we receive rabbits at 10-12 weeks of age and we only house them for about 3 to 4 weeks, we decided to give group housing a try. Change is hard for most people and...

Rollin, B. E. 2012. ENRICHMENT as a moral presupposition of animal research. Enrichment Record 10, 13-16.

Enrichment is therefore not the bighearted largess that much of the research community seems to believe it is. Rather, it represents an obvious step towards creating a decent life for research animals and minimizing avoidable...

Maertens, L., Buijs, S., Davoust, C. 2012. Gnawing blocks as cage enrichment and dietary supplement for does and fatteners: Intake, performance and behaviour. World Rabbit Science 21(S1), 185-192.

The aim of this study was to evaluate different experimental gnawing blocks as cage enrichment in rabbits. One hundred and five pregnant rabbit does housed in conventional wire cages were distributed according to their parity...

Bratcher, N., Clemons, D., Medina, L. 2012. History of enrichment in the ILAR Guide for the Care and Use of Laboratory Animals. Enrichment Record 12, 10-12.

Throughout the nearly 50 year history of the Institute for Laboratory Animal Research (ILAR) Guide, little attention or content was allocated to the subject of enrichment until the current (8th) edition (NRC 2011). Although animal...

Luchins, K. R., Baker, K. C., Gilbert, M. H. et al. 2011. Manzanita wood: A sanitizable enrichment option for nonhuman primates. JAALAS 50(6), 884-887.

Wooden objects are often used as nonhuman primate enrichment to provide variety and novelty, promote exploratory behavior, and supply an outlet for curiosity. However, concerns have been raised regarding the ability to sanitize wood by...

Buijs, S., Maertens, L., Tuyttens, F. A. M. et al. 2011. Glucocorticoid metabolites in rabbit faeces - Influence of environmental enrichment and cage size. Physiology & Behavior 104(3), 469-473.

The concentration of glucocorticoid metabolites (GCM) in rabbit faeces has been suggested as a non-invasive indicator of stress. In the present study, GCM concentrations were measured in faeces of fattening rabbits kept in groups of...

Poggiagliolmi, S., Crowell-Davis, S. L., Alworth, L. C. et al. 2011. Environmental enrichment of New Zealand white rabbits living in laboratory cages. Journal of Veterinary Behavior 6(6), 343-350.

The primary goal of environmental enrichment should be the avoidance of abnormal behaviors in laboratory animals such as rodents, lagomorphs, dogs, cats, and nonhuman primates. A total of 13 male single-housed New Zealand White rabbits...

Reinhardt, V. 2011. Home for Life - Sanctuaries for Abandoned Pets. Viktor Reinhardt, Mt. Shasta.

This book focuses primarily on human kindness and compassion rather than on human cruelty, callousness, indifference, and egocentric endeavors. It is common knowledge that many lay people and professional researchers treat animals in their charge...

Veillette, M., Reebs, S. G. 2011. Shelter choice by Syrian hamsters (Mesocricetus auratus) in the laboratory. Animal Welfare 20(4), 603-611.

The preference of Syrian hamsters (Mesocricetus auratus) for different in-cage shelters was tested. First, 15 males and 15 females were made to choose between a cage with a shelter and one without. Different shelters were...

Research Department, RSPCA. 2011. Rabbits: Good Practice for Housing and Care (3rd edition).

This report describes what rabbits need in terms of social housing, enclosure size and type, and environmental enrichment. It also describes potential husbandry-related welfare problems and how to resolve them.

Špinka, M., Wemelsfelder, F. 2011. Environmental challenge and animal agency. Animal Welfare 27–43.

Challenges are there to be overcome – seen usually as problems to avoid rather than as opportunities to enjoy. However, for humans a life without challenge would be likely to be dull and boring, lacking...

Andrews-Kelly, G. 2010. Canine socialization through the use of playrooms or exercise rooms. Enrichment Record 5, 7-9.

Comprehensive enrichment programs for dogs, while not required by regulations, are becoming more common in research facilities. Many institutions are providing chewable enrichment devices and pair or group housing for their canine colonies. Human interaction...

Hoy, J. M., Murray, P. J., Tribe, A. 2010. Thirty years later: Enrichment practices for captive mammals. Zoo Biology 29(3), 303-316.

Environmental enrichment of captive mammals has been steadily evolving over the past thirty years. For this process to continue, it is first necessary to define current enrichment practices and then identify the factors that limit...

Williams-Fritze, M. J., Altieri, J. A., Clough, C. et al. 2010. Modification of a dog cage for use as an exercise unit for rabbits. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 703 (Abstract #P70).

The Guide for the Care and Use of Animals and the USDA Animal Welfare Regulations (AWR) outline minimum space requirements for laboratory animals, including rabbits. These documents encourage housing of animals in enclosures that allow...

Vyas, D., Mayfield, K. L. 2010. Visual aid for creating enrichment items. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 705 (Abstract #P76).

Enrichment devices are administered to laboratory animals to reduce abnormal behaviors and to promote species typical behaviors (for example, foraging and nest building). With a diverse collection of species that require numerous enrichment options, a...

Veillette, M., Reebs, S. G. 2010. Preference of Syrian hamsters to nest in old versus new bedding. Applied Animal Behaviour Science 125(3-4), 189-194.

This study tested whether Syrian hamsters (Mesocricetus auratus) have an aversion to old bedding (up to 14 days) by offering them the option to nest in a new cage. A secondary goal was to assess...

Shulder, L., Ogbin, J. 2010. Zen Pen. Enrichment Record 5, 8.

At Huntingdon Life Sciences, we have developed a program to meet the exercise and socialization needs of our canines. Our program, entitled "Zen Pen" is a 30-minute weekly play session with at least two technicians...

Pullen, A. J., Merrill, R. J. N., Bradshaw, J. W. S. 2010. Preferences for toy types and presentations in kennel housed dogs. Applied Animal Behaviour Science 125(3-4), 151-156.

Toys are often provided for adult dogs housed in kennels, but their effectiveness as environmental enrichment is not well documented. At a minimum, toys need to elicit interest in the animal for which they are...

Mayfield, K. L., Livingston, L. G., Kirby, D. W. 2010. Floor housing rabbits to promote weight loss and species-specific behaviors. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 706-707 (Abstract #P81).

Standard rabbit caging offers little in the way of exercise, vertical space for species-typical movements (that is, standing on hind limbs), or ample space for proper social introductions. One commonly used rabbit breed, the New...

Lofgren, J. L., Wrong, C., Hayward, A. et al. 2010. Innovative social rabbit housing. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 659 (Abstract #PS13).

While female rabbits can be safely cohoused, aggression and resulting fight wounds preclude cohousing males. However, males may still benefit from social enrichment. Currently no housing systems provide males a safe opportunity for social interaction...

Lett, G. S. 2010. Measuring the impact of animal enrichment on research results. Enrichment Record 2, 6-7.

My take on this message is that the research community is gathering enough data from laboratory animals under a variety of environmental conditions, but we are not recording and/or sharing enough information about the animal...

Lett, G. S., Patterson-Kane, E. G. 2010. Environmental enrichment fights cancer and improves research results - What now for the biomedical researcher? Enrichment Record 5, 4-6.

The article, “Environmental and Genetic Activation of a Brain-Adipocyte BDNF/Leptin Axis Causes Cancer Remission and Inhibition,” was first published in Cell on July 8, and quickly echoed by online editions of major journals including The...

Howard, B., Nevalainen, T., Perretta, G. (ed). 2010. The COST Manual of Laboratory Animal Care and Use: Refinement, Reduction, and Research (1st Ed). CRC Press, Boca Raton, FL.

COST Action B-24 established four working groups to research and discuss issues relevant to laboratory animal science. These included the housing of animals, environmental needs, refinement of procedures, genetically modified animals, and cost-benefit analysis. Based...

Emmott, M. 2010. Covance animal environmental enrichment programme: In-house enrichment items, their benefits and the process of implementation. Animal Technology and Welfare 9, 189-190.

Some enrichment objects for rats, rabbits, dogs and macaques are demonstrated.

Edgar, J. L., Seaman, S. C. 2010. The effect of mirrors on the behaviour of singly housed male and female laboratory rabbits. Animal Welfare 19(4), 461-471.

It is widely recognised that single housing is detrimental to the welfare of social species. However, some experimental procedures dictate that laboratory animals are housed individually. There is evidence to suggest that, by mimicking social...