Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

The origins of floor-laying in ducks could be low motivation for a nest, or stress related to difficulties with accessing a nest (e.g. competition). Using a behavioural demand test, we investigated if increasing the work...

The zebrafish (Danio rerio) is widely used as a promising translational model organism for studying various brain disorders. Zebrafish are also commonly used in behavioral and drug screening assays utilizing individually tested (socially isolated) fish...

The concept of the 3Rs (Refinement, Reduction and Replacement) has been used as a framework for improving the welfare of laboratory animals for the last half century. By establishing an animal-centric view on housing and...

Good practice for the housing and care of laboratory zebrafish Danio rerio is an increasingly discussed topic, with focus on appropriate water quality parameters, stocking densities, feeding regimes, anaesthesia and analgesia practices, methods of humane...

Recent research has uncovered many complex cognitive traits and affective processes in many reptile species, such as the ability to make choices that are rewarding or pleasurable. The investigation herein was initiated after many years...

The effect of environmental enrichment on the behavior and welfare in captivity of reptiles and of freshwater turtles in particular, which are popular aquarium and pet species, is very little studied compared to other taxa...

Appropriate management of social groups is one of the greatest challenges that face zoos and aquaria worldwide. To facilitate breeding programmes, particularly in polygynous species, there is a need to house surplus males in bachelor...

Environments that do not provide animals with appropriate stimuli may affect their welfare and reproduction, for example by precluding them from exhibiting their full behavioural repertoire. In this study, we sought to evaluate the effectiveness...

Wildlife research has been indispensable for increasing our insight into ecosystem functioning as well as for designing effective conservation measures under the currently high rates of biodiversity loss. Genetic and genomic analyses might be able...

Environmental enrichment is used to increase social and physical stimulation for animals in captivity which can lead to enhanced cognition. Fundamental to the positive effect enrichment has on the brain is that it provides opportunities...

Temperament assessment is useful in reintroduction programs. Reactivity to humans and flight ability are also important behavioral aspects for captive parrots candidates to reintroduction. The study aimed: a) to evaluate if behavioral responses to environmental...

Growing research effort has shown that physical enrichment (PE) can improve fish welfare and research validity. However, the inclusion of PE does not always result in positive effects and conflicting findings have highlighted the many...

Recent studies showed that freshwater turtles display inter-individual differences in various behavioural traits, which may influence their health and welfare in captivity due to differences in response to husbandry and enrichment strategies and in ability...

Environmental enrichment is a common tool used in zoo and aquarium settings to provide mental and physical stimulation to animals under human care. This paper aims to present a primary approach to goal-oriented environmental and...

Cetaceans are long-lived, social species that are valued as ambassadors inspiring the public to engage in conservation action. Under professional care, they are critical partners with the scientific community to understanding the biology, behavior, physiology...

The way an animal uses its habitat can serve as an indicator of habitat appropriateness for the species and individuals. Bottlenose dolphins (Tursiops truncatus and Tursiops aduncus) in accredited zoos and aquariums experience a range...

Environmental enrichment supports the well-being and welfare of captive animals. In the current study, the most suitable form of enrichment device for captive green turtles (Chelonia mydas) was investigated, to support head-start programs rearing turtles...

Captive welfare studies in odontocete species have been mostly conducted on bottlenose dolphins (Tursiops truncatus) while the welfare of many other species’ -including endangered species- remains poorly studied. More research is needed to find and...

Zebrafish has become an animal model in research and articles have established ideal conditions for their maintenance. However, little is known regarding the influence of gender and other cues on zebrafish behavior. Thus, here we...

Cognitive enrichment aims to provide animals with opportunities to use their cognitive skills and to promote behaviors associated with positive wellbeing. Cooperation in mammals has been recorded during various behavioral contexts such as hunting, mating...

Environmental enrichment is a key component in improving the psychological and physiological well-being of animals under professional care. Environmental enrichment involves the addition of stimuli, including objects and cognitive challenges, into the environment in order...

Despite the increasing popularity of zebrafish (Danio rerio) as an animal model, the environmental enrichment preferences of this species have been largely unexplored. We sought to determine the preferences of mature female zebrafish that were...

Studies on the etiology of behavioral problems often involve interference in the animal's routine or reliance on owners' self-reports like surveys. Gathering data from videos posted on social media, a technique coined ‘video mining’, offers...

Anthropogenic change is expected to alter environments at alarming rates. To predict the impact of modified environments on social behavior, we must study the relationship between environmental features and collective behavior in a genetically tractable...

Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and...

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