Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 
General anesthesia as used for rodent research can have adverse effects on physiologic mechanisms. Thermoregulation is often greatly inhibited, with resultant deleterious effects on cardiac and respiratory function. These potential effects can be mitigated by...
In aquaculture, to ensure animal welfare in pre-slaughter and slaughter stages, it is fundamental that fish are insensible. A method for evaluating fish insensibility is based on visual sensibility indicators (VSI) assessment (i.e., self-initiated behavior...
Hamsters have historically been used in our pharmacokinetic (PK) studies using the retro-orbital (RO) bleeding technique to collect blood samples. If performed incorrectly, this technique has the potential for animal welfare complications not usually seen...
Research in ecology and wildlife biology remains crucial for increasing our knowledge and improving species management and conservation in the midst of the current biodiversity crisis. However, obtaining information on population status often involves invasive...
Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non‐invasive method for obtaining...
Individual animal personalities affect experiences of zoo environments, and thus potentially welfare. Incorporating keeper knowledge of animal personality in a reliable way has great value in optimising welfare in zoo-housed animals. Assessment of animal personality...
The animals contained within the phylum Cnidaria (Sea Anemones, Corals, Sea Pens, Jellyfish, Boxjellies and Hydra) have origins that can be dated back to around 750 million years ago (mya) and as such, they represent...
The revised fifth edition of Clinical Laboratory Animal Medicine: An Introduction is an accessible guide to basic information for conducting animal research safely and responsibly. It includes a review of the unique anatomic and physiologic...
Personality, defined as consistent between-individual variation in clusters of behavioral traits independent of factors such as age or sex, emerges in most animal species tested so far. The number of invertebrate species discovered to have...
Spiders with around 48,000 recorded species are major terrestrial predators and thus crucially important for ecosystem functioning. They are widely used as research models and for biodiversity displays and sometimes also kept as pets. Nevertheless...
Much of the content in the 2018 Code is similar to that found in previous guidance documents: the 2002 Code of Practice for the Welfare of Meat Chickens and Meat Breeding Chickens, and the 2011...
Repeated injections in rats and mice are typically done via the tail vein. For hamsters, the tail is not an option. In this paper we explore the development and refinement of IV dosing in the...
Rainbow trout Oncorhynchus mykiss were infiltrated with either saline or lidocaine adjacent to the dorsal fin to assess histopathological changes. Infiltration was done as if it were being used as a local anaesthetic. Tissue lesions...
Laboratory animals are still necessary in scientific investigation and vaccine testing, but while novel methodological approaches are not available for their replacement, the search for new, humane, easy, and painless methods is necessary to diminish...
In the US, the Animal Welfare Act (AWA) and its enabling regulations (AWAR) cover all warm-blooded animals used for research, testing, experimentation, or exhibition. The only exceptions, made in the enabling regulations, are for two...
Specific positioning for different imaging modalities is essential in our small animal imaging core. While we have different animal holders designed to position the animal inside the MRI, additional securing of the animal to the...
Clinical Laboratory Animal Medicine: An Introduction, Fourth Edition offers a user-friendly guide to the unique anatomy and physiology, care, common diseases, and treatment of small mammals and nonhuman primates. Carefully designed for ease of use...
These guidelines aim to provide information for investigators, animal care committees, facility managers and animal care staff that will assist in improving both the care given to farm animals and the manner in which experimental...
This article provides details to consider when preparing to use animals in biomedical research. The stress of transport and receipt of animals into a new environment mandate the need for a period of stabilization and...
The question was asked: "In your own experience, what is the least disturbing technique - from the subject's point of view! - of blood collection for rodents?" In summary, it is fair to conclude the...
In this article, laws and guidelines relating to rodent enrichment are reviewed, the natural behaviors of select rodent species are discussed, and an overview of widely used types of enrichment in laboratory rodent management is...
Expanding on the National Research Council’s Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of...

Share This!