Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 
This article describes the strategies employed by Kumamoto Sanctuary in Japan to integrate 3 retired chimpanzees – who each spent 30 years singly housed in a biomedical facility – with the sanctuary’s other residents.
The soft‐bodied cephalopods including octopus, cuttlefish, and squid are broadly considered to be the most cognitively advanced group of invertebrates. Previous research has demonstrated that these large‐brained molluscs possess a suite of cognitive attributes that...
In conclusion, the investigations carried out up to now demonstrate that during the early stages fish show high sensitivity to many types of stressors involving an array of responses to overcome alterations that could affect...
Animal welfare is one of the most challenging issues in modern farm animal husbandry. Animal welfare indicators can be used to monitor welfare on farms or at slaughterhouses, with footpad dermatitis (FPD) being one of...
This study aimed to determine the prevalence of keel bone deviations and feather damage of laying hens in two different free-range housing systems under commercial conditions. Both of the free-range systems had an indoor barn...
Gait defects, assessed as gait score (GS), is a common welfare problem in broiler chicken production. The aim of this study was to examine whether birds with GS2 experience pain and poor locomotor ability compared...
This 30-chapter volume informs students and professionals about the behavioral biology of animals commonly housed in laboratory and other captive settings. Each species evolved under specific environmental conditions, resulting in unique behavioral patterns, many of...
Early-life experiences may considerably affect the behavioural patterns of adult primates. Particularly, atypical rearing practices might lead to abnormal behaviours and social-sexual deficiencies in captive, adult non-human primates. We conducted behavioural observations of mother-reared (n...
Fractures and deviations to the keel bone are common in commercial laying hens, with reported variations in occurrence across strains and breeds. The aetiology is not fully understood, however, modern genetics and selection for efficient...
Behavioural diversity may serve as a positive indicator of animal welfare that can be applied in long-term monitoring schemes in managed settings (eg zoos, laboratories, farms). Behavioural diversity is often higher when animals live in...
The objective was to determine broiler chicken behavioral differences in response to novel flooring treatments. Broilers (n = 182) were housed in 14 pens (a random subset from a larger-scale study including 42 pens), with...
Comparative thanatology encompasses the study of death-related responses in non-human animals and aspires to elucidate the evolutionary origins of human behavior in the context of death. Many reports have revealed that humans are not the...
For over a century, the practice of deflighting has taken place in zoological collections in order to ensure birds remain in open-topped enclosures. Over time, efforts have been made to improve or develop new (surgical)...
In this study, an appropriate visual scoring system for foot-pad dermatitis was validated, considering the histologically measured depth of the inflammation zone and the histopathological grade (no lesion, mild lesion, ulcer). The aim being to...
Reduced mobility in broilers can contribute to leg health problems. Environmental enrichment has been suggested as one approach to combat this through stimulating increased physical activity. Past studies have tested the effect of environmental enrichments...
Factors contributing to the development of keel bone damage are not well understood. This study aimed to identify behaviors and cage structures associated with acceleration events experienced by individual hens at their keels as the...
Optimising nest design for broiler breeders has benefits for both the animals and the producers. The welfare of the hens will increase by providing preferred housing, while also reducing eggs laid outside the nests. These...
Broiler breeders are feed-restricted during rearing to avoid the negative consequences of obesity during lay due to their genetic potential for fast growth rate. Feed restriction leads to chronic hunger, frustration and lack of satiety...
Keel bone fractures in laying hens have been described with increasing prevalence from several countries over the last twenty years and are considered one of the greatest welfare problems to the layer industry. In Denmark...
In commercial flocks of laying hens, keel bone fractures (KBFs) are prevalent and associated with behavioural indicators of pain. However, whether their impact is severe enough to induce a depressive-like state of chronic stress is...
While non-human primate studies have long been conducted in laboratories, and more recently at zoological parks, sanctuaries are increasingly considered a viable setting for research. Accredited sanctuaries in non-range countries house thousands of primates formerly...
Chimpanzees in zoos with sufficient and appropriate environmental enrichment devices are expected to exhibit behaviors, interactions, and societies similar to those in the wild. In this study, we compared the activity budgets of each observed...
Failing to provide hamsters with a method to wear down their teeth can lead to detrimental behaviour such as bar chewing, and health issues, for example, overgrown teeth and tooth loss. However, hamsters use their...
Endangered whooping cranes (Grus americana ) have been produced in captivity for reintroduction programs since the 1980s, using techniques such as artificial insemination, multiple clutching, and captive‐rearing to speed recovery efforts. Chicks are often hand‐reared...
Chickens cannot independently thermoregulate at hatch and lack opportunity to behaviorally thermoregulate with a hen in the egg layer industry, thus barns are heated to thermoneutral temperatures. Dark brooders are low-energy-consuming hot plates, which may...

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