Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

Automatic feeders have already been developed by different institutions and companies. Feedpods are a perfect example of an elaborate manufactured device. They are currently used in many institutions with satisfying results. One of the main...

Captive animal welfare is important for establishments that exhibit species for education, conservation, and research. However, captive animals are often exposed to a number of potential stressors, such as visitors and anthropogenic noise. We aimed...

Evaluations of enrichment are critical to determine if an enrichment program is meeting stated goals. However, nocturnal species can present a challenge if their active periods do not align with caretakers’ schedules. To evaluate enrichment...

A protocol for photo‐identification of individual Megatrygon microps has been defined. One hundred and four identification photographs were taken between 2005 and 2019. Spot patterns on the dorsal surface were used to identify individuals. Unique...

This article describes the steps for making a DIY guinea pig or rabbit cardboard foraging ball. Pictures are provided for each step as well as the completed ball. These cardboard foraging balls can be made...

In semi-natural mixed-sex groups, rabbits (Oryctolagus cuniculus) organize their social life by forming a hierarchy, which is characterized by linearity and stability. Compared to the natural environment, many factors are different in contemporary breeding systems...

The present study assessed the diurnal variation in salivary cortisol in captive African elephants during routine management (baseline) and in relation to a potential stressor (translocation) to evaluate to what extent acute stress may affect...

For many species in zoos, particularly megafauna vulnerable to heat stress, shade is a key environmental resource. However, shade availability has received comparatively less attention than other aspects of the zoo environment. In this study...

The aim of this study was to develop and test a tilapia on-farm welfare assessment protocol, based on Brazilian semi-intensive production systems. The study included two mains steps: the elaboration of tilapia welfare protocol and...

Salivary immunoglobulin A (sIgA) has been proposed as a potential indicator of welfare for various species, including Asian elephants, and may be related to adrenal cortisol responses. This study aimed to distinguish circadian rhythm effects...

OBJECTIVE: To evaluate antinociceptive efficacy and safety of SC buprenorphine hydrochloride administration in African pygmy hedgehogs (Atelerix albiventris). ANIMALS: 12 healthy adult hedgehogs (7 males and 5 females). PROCEDURES: 3 crossover experimental trials were performed...

Cognitive enrichment aims to provide animals with opportunities to use their cognitive skills and to promote behaviors associated with positive wellbeing. Cooperation in mammals has been recorded during various behavioral contexts such as hunting, mating...

The aim of the study was to assess the effect of two different stunning methods on the level of blood stress indicators (cortisol, insulin, glucose) and rabbit meat quality. The experiment was conducted on crossbreds...

Pathological lesions of feet occur frequently in captive elephant populations. To improve foot health, it is important to identify risk factors associated with such pathologies. Several previous studies have analyzed potentially influencing factors but were...

Environmental enrichment is an important tool utilized to improve animal welfare in zoological institutions through opportunity for mental and physical stimulation. Many past studies have focused on the impact enrichment has on animal behavior; however...

Comprehensively explains animal learning theories and current best practices in animal training within zoos. This accessible, up-to-date book on animal training in a zoo/aquaria context provides a unified approach to zoo animal learning, bringing together...

Knowledge of the welfare status of wild animals is vital for informing debates about the ways in which we interact with wild animals and their habitats. Currently, there is no published information about how to...

Plasma lipid and glucose levels are important parameters for evaluating the onset and development of metabolic and cardiovascular diseases. In clinical and experimental studies of humans or mice, fasting is often required before testing plasma...

Zoos play an important role in conservation, participating in captive breeding programmes and scientific research. The research can be shared and inform practice in the field and zoos. A behavioural observation study was conducted on...

The Becton Dickinson (BD) Research Animal Care Program has developed an acclimation and enrichment program that has enhanced animal welfare for studies requiring single housing, contributed to a culture of care, increased transparency, and improved...

In the wild, hippopotamuses spend much of their daily activity in the water. In zoos, it is less clear the extent to which hippos spend time in the water. We examined how much time Woodland...

The ecology of large, wide-ranging carnivores appears to make them vulnerable to conservation challenges in the wild and welfare challenges in captivity. This poses an ethical dilemma for the zoo community and supports the case...

Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non‐invasive method for obtaining...

The welfare status of elephants under human care has been a contentious issue for two decades or more in numerous western countries. Much effort has gone into assessing the welfare of captive elephants at individual...

Information regarding the physiology of African lions is scarce, mainly due to challenges associated with essential routine research procedures. The aim of this experiment was to test the possibility of training six captive lionesses by...

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