Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

Husbandry procedures and facility settings, such as low-frequency fire alarms, can produce noises in a laboratory environment that cause stress to animals used in research. However, most of the data demonstrating harmful effects that have...

Given the paucity of published data on the effects of artificial weaning on mares, the aim of the present study was to investigate the behavioral and hormonal effects of two different weaning methods in trotter...

This study analyzed the rectal and surface temperature of the horse's body, its heart rate variability parameters, and the step length when walking and trotting geriatric horses using blankets with various thermoinsulating properties. Twenty-four hot-blooded...

Exposure to environmental factors such as high temperatures and solar radiation levels present a welfare concern for many domestic equids. Understanding how these factors influence the shelter use of healthy equids can inform welfare guidelines...

Pet rabbit welfare is a hidden crisis: Inappropriately housed, fed, and not routinely provided healthcare—rabbits can often suffer painful conditions and shortened lifespans. This study provides novel understanding of owners’ perceptions of rabbits’ mental capabilities...

This review focuses on associations of cortisol and the hormone ghrelin on abnormal oral behaviors, predominantly stereotypic behavior, in horses. Abnormal oral behaviors are prevalent in the stabled horse population. Feeding practice and satiety seem...

During the evolution of the horse, an extended period of feed intake, spread over the entire 24-h period, determined the horses’ behaviour and physiology. Horses will not interrupt their feed intake for more than 4...

The most widespread reproductive rhythm practiced in rabbit farming is based on artificial insemination (AI) performed at around 11 days pospartum. This approach results in high production rates, but requires high yearly replacement of animals...

Grazing muzzles are a popular and effective management tool used to help prevent weight gain in obese-prone equids. Concerns have been raised over their possible negative impact on horse well-being as muzzles may interfere with...

This is the 5th volume of selected discussions that took place on the electronic Laboratory Animal Refinement & Enrichment Forum between February 2016 and December 2019. The forum was created in October 2002; it allows...

Grazing muzzles are used on obese-prone equids to limit intake of pasture while grazing. However, the length of time grazing muzzles must be worn to prevent weight gain and whether they impede normal grazing behavior...

Plasma lipid and glucose levels are important parameters for evaluating the onset and development of metabolic and cardiovascular diseases. In clinical and experimental studies of humans or mice, fasting is often required before testing plasma...

Horse confinement is a common practice worldwide. However, preventing horses from grazing and denying them social interaction can compromise their welfare and lead to undesirable consequences. Considering the hypothesis that increasing the forage consumption time...

In an effort to minimize the amount of hay wasted from a round bale, horse owners generally surround the bale with a round-bale feeder. While often necessary as a supplemental feed source, this feeding technique...

The RSPCA/UFAW Rodent (and now Rabbit) Working Group has held a one-day meeting every autumn for the last 25 years, so that its members can discuss current welfare research, exchange views on welfare issues and...

This second edition is fully revised, expanded, and comprehensively updated with the most current knowledge about the full array of mental health issues seen in animals. Written by key opinion leaders, internationally-recognized experts and specialists...

Preferences for the height of feeding troughs of seven mares were evaluated and, as awareness of surroundings when feeding is adaptive for horses, the relationship between preference responses and attention behaviours was also assessed. First...

Despite the spatial and social restrictions it causes, single stall housing still prevails in sport and riding school horses, leading to the emergence of abnormal behaviours such as stereotypic or abnormal repetitive behaviours (SB/ARB). In...

Horses use human-made shelters actively during inclement weather, but the costs of building shelters may be high and owners use blankets or rugs on horses instead. The aim of the study was to investigate how...

“Pheromonal therapy” has been promoted as a promising alternative therapy to improve the human-animal relationship and to reduce behavioral reactions to stressful stimuli. This placebo-controlled double blind study evaluated the use of a synthetic equine...

Horses are mainly housed in individual boxes. This housing system is reported to be highly detrimental with regard to welfare and could trigger the expression of four behavioural indicators of a compromised welfare state: stereotypies...

When environmental temperatures exceed 25°C, horses are potentially subjected to thermal stress. It has therefore been recommended that horses should be provided with shade during hot days. However, this is not possible for horses grazing...

Past research has indicated that horse hooves change shape in response to gallop exercise, so this study sought to separate the changes due to exercise from changes due to wearing horseshoes. This crossover study tested...

Data are available in the scientific literature concerning the quality and usefulness of donkey milk for human consumption. However, there is a lack of studies related to the understanding of the welfare of dairy donkeys...

Icelandic horses are often stabled in loose-housing systems, and to date this type of stabling has not been evaluated with regard to its potential impact on respiratory health. The objective was to assess if differences...

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