Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

The lighting spectrum used in modern poultry production commonly lacks significant amounts of ultraviolet light; however, poultry can perceive the ultraviolet spectrum unlike humans and little research has been done to investigate the importance of...

The feed restriction applied during rearing of broiler breeders inflicts chronic hunger, and frustration due to unfulfilled behavioural needs for feeding. To alleviate the welfare problems associated with feed restriction, qualitative feed restriction allows a...

Dairy calves must be fed appropriately to meet their nutritional needs, supporting optimal growth and development to achieve the recommended target age at first calving (AFC) of 24 months. Traditional restricted milk feeding practices suppress...

Due to its increasing pressure on dairy cows, studies that investigate how to cope with heat stress are needed. The heat stress affects multiple aspects of cows’ lives, among which their behavior and welfare. In...

The aim of this study was to determine the effects of two housing systems (thermal plastic greenhouse roofs—PGR—vs. without roof—PWR) on health, welfare, and finishing performance of zebu steers in cold arid environments. A total...

Threshold temperatures for cold stress in sheep are not well understood, the available literature is somewhat dated and reports relate to winter temperatures that are relatively benign. Sheep’s preferences for outdoor versus indoor environments, when...

Beak trimming in laying hens limits the negative consequences of injurious pecking, but could be prohibited by future regulations. This study assessed a combination of management strategies during the rearing period (objects, perches, music, human...

Plasma lipid and glucose levels are important parameters for evaluating the onset and development of metabolic and cardiovascular diseases. In clinical and experimental studies of humans or mice, fasting is often required before testing plasma...

Sheep lameness is a major concern among farmers and policymakers with significant impacts on animal welfare standards as well as financial and production performance. The present study attempts to identify the relative importance of environmental...

A widespread management practice in commercial dairy farms is to stop milk production artificially commonly 2 months before expected calving, in a process termed drying-off. Dry-off management normally encompasses dietary changes as well as abrupt...

In the wild, excreta soiled surroundings can attract predators and spread disease. Yet, farmers rear broiler chicks in large barns with stocking densities that prevent excreta segregation. To measure chicks’ motivation to access unsoiled bedding...

First-week mortality is an important performance index as well as an important welfare indicator. The aim of the present study was to identify internal (individual-dependent) and external (management or environmental) factors that could influence the...

In hot arid and semi-arid regions, heat stress is a major constraint in animal production due to prolonged summers with high temperatures. In dairy husbandry, the desert cooler and evaporative cooling system are effectively used...

Weaning from milk is stressful. We aimed to investigate the effect of three weaning strategies on feed intake and behavior of dairy goat kids. Forty-five, 3-month-old Saanen cross goats were allocated to one of three...

Chickens cannot independently thermoregulate at hatch and lack opportunity to behaviorally thermoregulate with a hen in the egg layer industry, thus barns are heated to thermoneutral temperatures. Dark brooders are low-energy-consuming hot plates, which may...

Previous studies have demonstrated that dairy cattle prefer pasture over indoor conditions especially at nighttime, which suggests they perceive pasture as an attractive place to rest. Indeed, cows avoid and spend less time lying on...

Natural light with ultraviolet spectrum (UV) influences the birds´ perception, the reflectivity of their plumage and affects bird behavior. Therefore, in Germany, laying hens kept in barns should be provided with daylight inlets. Nevertheless, lighting...

Assessing animal welfare is necessary from animal behavior and product quality/quantity perspectives. Birds have a unique visual system and see in the range of 315-750 nm. Most of their behavior is mediated by vision. Lighting...

Chickens under appropriate light–dark programs can develop diurnal rhythms. However, actual needs of layer pullets for the light and dark environments are not fully understood. This study was to investigate the diurnal rhythms of pullets...

Ruminants evolved in diverse landscapes of which they utilized, by choice, a diverse arrangement of plants (grasses, forbs, and trees) for food. These plants provide them with both primary (carbohydrates, protein, etc.) and secondary (phenolics...

Mechanical brushes allow cows to groom. Mange is common in indoor-housed cattle, and this affliction may affect cow motivation to use a mechanical brush. The aim of this study was to assess changes in dairy...

Under intensive rearing conditions, the welfare of broiler chickens may be at risk depending on genotype and sex, due to their different growth rates. The practice of quantitative feed restriction may also impact on welfare...

In order to assess the level of sheep welfare in small traditional farms as compared with farms of increased size we evaluated several animal-based parameters and applied a modified Animal Needs Index (ANI) protocol in...

Broiler breeders are feed-restricted during rearing to avoid the negative consequences of obesity during lay due to their genetic potential for fast growth rate. Feed restriction leads to chronic hunger, frustration and lack of satiety...

Pasture access for dairy cows is highly valued both by cows and the public at large. When pasture access is not feasible, farmers can provide cows with alternative forms of outdoor access, such as an...

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