Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
Robinson, L. M., Weiss, A. (Eds.) 2023. Nonhuman Primate Welfare: From History, Science, and Ethics to Practice. Springer, Cham, Switzerland. 671 p.

This volume reviews the broad topic of welfare in nonhuman primates under human care. Chapters detail the history of primates in captivity, ethical and legal issues surrounding the use of nonhuman primates as entertainment or...

Ausderau, K. K., Colman, R. J., Kabakov, S. et al. 2023. Evaluating depression- and anxiety-like behaviors in non-human primates. Frontiers in Behavioral Neuroscience 16, 1006065.

Depression and anxiety are some of the most prevalent and debilitating mental health conditions in humans. They can present on their own or as co-morbidities with other disorders. Like humans, non-human primates (NHPs) can develop...

Bradshaw-Wiley, E., Randle, H. 2023. The effect of stabling routines on potential behaviour indicators of affective state in horses and their use in assessing quality of life. Animals 13(6), 1065.

Increasing interest in equine welfare has emphasised the need for objective and reliable behavioural indicators of horses’ affective state. However, research has yielded mixed results regarding behaviours suited for industry use largely because they are...

Cohen, S., Ho, C. 2023. Review of rat (Rattus norvegicus), Mouse (mus musculus), Guinea pig (Cavia procellus), and rabbit (Oryctolagus cuniculus) indicators for welfare assessment. Animals 13(13), 2167.

The monitoring and assessment of animals is important for their health and welfare. The appropriate selection of multiple, validated, and feasible welfare assessment indicators is required to effectively identify compromises or improvements to animal welfare...

Ferlini Agne, G., May, B. E., Lovett, A. et al. 2023. Horse grimace scale does not detect pain in horses with equine gastric ulcer syndrome. Animals 13(10), 1623.

Equine gastric ulcer syndrome (EGUS) is a highly prevalent and presumptively painful condition, although the amount of pain horses might experience is currently unknown. The aims of this study were to determine if the Horse...

Harvey, A. M., Beausoleil, N. J., Ramp, D. et al. 2023. Mental experiences in wild animals: Scientifically validating measureable welfare indicators in free-roaming horses. Animals 13(9), 1507.

The mental experiences of animals are what characterises their welfare status. The Five Domains Model for assessing welfare aligns with the understanding that physical and mental states are linked. Following measurement of indicators within each...

Kumar, P., Abubakar, A. A., Imlan, J. C. et al. 2023. Importance of knife sharpness during slaughter: Shariah and Kosher perspective and scientific validation. Animals 13(11), 1751.

Halal and kosher slaughter have given the utmost importance to the sharpness of knives during the slaughter of animals. A sharp knife of appropriate dimension (blade length) makes slaughter less painful during neck severance and...

Padalino, B., Benedetti, B., Felici, M. et al. 2023. GLAD scale for ranking welfare of horses on arrival after transport to slaughterhouses. Animals 13(9), 1465.

To date, there is no official method for measuring horse welfare after transport. This study aimed to develop a scale to classify horses into four categories: good shape; light affected; affected; down (GLAD) based on...

Seganfreddo, S., Fornasiero, D., De Santis, M. et al. 2023. A pilot study on behavioural and physiological indicators of emotions in donkeys. Animals 13(9), 1466.

Recognizing animal emotions is critical to their welfare and can lead to a better relationship with humans and the environment, especially in a widespread species like the donkey, which is often prone to welfare issues...

Silva-Reis, R., Faustino-Rocha, A. I., Silva, J. et al. 2023. Studying and analyzing humane endpoints in the fructose-fed and streptozotocin-injected rat model of diabetes. Animals 13(8), 1397.

This work aimed to define a humane endpoint scoring system able to objectively identify signs of animal suffering in a rat model of type 2 diabetes. Sprague-Dawley male rats were divided into control and induced...

Swan, J., Boyer, S., Westlund, K. et al. 2023. Decreased levels of discomfort in repeatedly handled mice during experimental procedures, assessed by facial expressions. Frontiers in Behavioral Neuroscience 17, 1109886.

Mice are the most commonly used laboratory animal, yet there are limited studies which investigate the effects of repeated handling on their welfare and scientific outcomes. Furthermore, simple methods to evaluate distress in mice are...

Lamon, T. K., Slater, M. R., Moberly, H. K. et al. 2023. Welfare and quality of life assessments for shelter cats: A scoping review. Applied Animal Behaviour Science 258, 105797.

The aim of this scoping review was to provide an overview of the published welfare and quality of life assessments that are available for shelter cats. The specific objectives were to identify the available assessments...

Machado, M., Freitas Silveira, R. M., Machado Bittar, C. M. et al. 2023. Can the emotional state of calves be noticed by their facial expression and heart rate? Applied Animal Behaviour Science 260, 105874.

The aim of this study was to identify whether or not dairy calves change their facial expression and heart rate according to their emotional state when subjected to a stroking or umbrella stimulus. Thirty-two mixed-breed...

Zuliani, L., Esteves Trindade, P. H., Rodrigues Paranhos da Costa, M. J. 2023. Welfare assessment of horses and mules used in commercial beef cattle ranches in Brazil. Applied Animal Behaviour Science 264, 105964.

This study aimed to identify, among the equine welfare indicators included in two available protocols (Assessment Protocol for Horses and AWIN - Welfare Assessment Protocol for Horses), those suitable to assess the welfare of ranch...

Ninomiya, S., Goto, Y., Huricha et al. 2023. Lying posture as a behavioural indicator of heat stress in dairy cows. Applied Animal Behaviour Science 265, 105981.

Dairy cows tend to stand as a heat stress response, but the increased tendency to stand adversely affects their productivity and welfare. Thus, taking measures against heat stress before cows spend more time standing is...

Ricci-Bonot, C., Mills, D. S. 2023. Recognising the facial expression of frustration in the horse during feeding period. Applied Animal Behaviour Science 265, 105966.

Horses often present negative emotional states which are frequently poorly recognised, with much of our understanding of horse expressions based on anecdotes, rather than scientific evidence. The aim of this project was to identify potential...

Moëne, O. L., Larsson, M. 2023. A new tool for quantifying mouse facial expressions. ENeuro 10(2) ENEURO.0349-22.2022.

Facial expressions are an increasingly used tool to assess emotional experience and affective state during experimental procedures in animal models. Previous studies have successfully related specific facial features with different positive and negative valence situations...

Palacio, S., Zambelis, A., Adam, S. et al. 2023. Reassessing cow comfort measures on Canadian dairy farms after a recommendation of improvements. Canadian Journal of Animal Science 103(2), 174–184.

In a previous study (Assessment 1), an on-farm assessment tool was used to establish a standard across 60 Quebec dairy farms using animal-, stall-, and management-based measures of cow comfort. The objective of the present...

Ataallahi, M., Cheon, S. N., Park, G.-W. et al. 2023. Assessment of stress levels in lactating cattle: Analyzing cortisol residues in commercial milk products in relation to the temperature-humidity index. Animals 13(15), 2407.

Chronic stress in the dairy cattle industry has negative impacts on animal health, productivity, and welfare. It has been confirmed that cortisol transfers to milk and resists the high temperature during milk processing. This study...

Bukhari, S. S. U. H., Parkes, R. S. V. 2023. Assessing the impact of draught load pulling on welfare in equids. Frontiers in Veterinary Science 10.

About 112 million working equids are the source of income for 600 million people globally. Many equids are used for pulling loads (up to 15,000 kg per day) to transport goods. Most of them are associated...

Dietrich, T., Aigner, A., Hildebrandt, A. et al. 2023. Nesting behavior is associated with body weight and grip strength loss in mice suffering from experimental arthritis. Scientific Reports 13(1), 23087.

Objective animal health evaluation is essential to determine welfare and discomfort in preclinical in vivo research. Body condition scores, body weight, and grimace scales are commonly used to evaluate well-being in murine rheumatoid arthritis (RA)...

Domínguez-Oliva, A., Hernández-Ávalos, I., Olmos-Hernández, A. et al. 2023. Thermal response of laboratory rats (Rattus norvegicus) during the application of six methods of euthanasia assessed by infrared thermography. Animals 13(18), 2820.

Refinement is one of the principles aiming to promote welfare in research animals. The techniques used during an experimental protocol, including euthanasia selection, must prevent and minimize suffering. Although the current euthanasia methods applied to...

Domínguez-Oliva, A., Olmos-Hernández, A., Hernández-Ávalos, I. et al. 2023. Rat grimace scale as a method to evaluate animal welfare, nociception, and quality of the euthanasia method of wistar rats. Animals 13(20), 3161.

Refinement of experimental procedures in animal research has the objective of preventing and minimizing pain/distress in animals, including the euthanasia period. This study aimed to evaluate pain associated with six methods of euthanasia in Wistar...

Dybdal, N., Horgan, M., Costa, L. et al. 2023. Equine gunshot euthanasia: Creation of a 3D-printed model with integrated sensors for training. Animals 13(16), 2566.

Challenges and issues related to the use of pentobarbital euthanasia and disposal of animal remains within the US have recently been reviewed. Environmental and public health challenges increasingly necessitate consideration of alternative methods such as...

Fletcher, K. A., Limon, G., Padalino, B. et al. 2023. Impact of social buffering and restraint on welfare indicators during uk commercial horse slaughter. Animals 13(14), 2276.

Current legislation in the United Kingdom stipulates that horses should not be slaughtered within sight of one another. However, abattoir personnel anecdotally report that, for semi-feral horses unused to restraint, co-slaughtering alongside a conspecific could...