Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 

Drug administration to experimental rodents is often invasive and stressful, thus reducing animal welfare and potentially confounding experimental results. Methods of oral drug delivery in which rodents cooperate voluntarily minimize stress, pain and morbidity. We herein...

The designer receptor exclusively activated by designer drugs (DREADD) system is one of the most widely used chemogenetic techniques to modulate the activity of cell populations in the brains of behaving animals. DREADDs are activated...

Recent studies at Liverpool University have indicated that handling mice by their tails during routine cleaning and procedures induced aversion and high anxiety in many commonly used strains. The evidence from the Liverpool study suggests...

Capybaras, also known as “water pigs,” are the world’s largest rodent. Although these animals are closely related to guinea pigs, with a shoulder height of approximately 2 ft, coarse brown fur, and partially webbed feet...

Physical touch can help to decrease the effects of stress. The aim of this study was to evaluate the influence of tactile stimulation on the hormonal and behavioral responses of young adult rats submitted to...

This article describes the authors’ experience with the housing, social housing, husbandry, handling, and environmental enrichment of chinchillas in laboratories.

The primary focus of this project was to introduce the low stress handling of mice to Newcastle University and resulting in all researchers and technicians using this method. It aimed to bring a team together...

Mice are the most commonly used laboratory animals for studying diseases, behaviour, and pharmacology. Behavioural experiment battery aids in evaluating abnormal behaviour in mice. During behavioural experiments, mice frequently experience human contact. However, the effects...

The RSPCA/UFAW Rodent Welfare Group has held a one-day meeting every autumn for the last 26 years, so that its members can discuss current welfare research, exchange views on welfare issues, and share experiences of...

Handling is a well-known source of stress to laboratory animals and can affect variability of results and even compromise animal welfare. The conventional tail handling in mice has been shown to induce aversion and anxiety-like...

Handling of laboratory mice is essential for experiments and husbandry, but handling can increase anxiety in mice, compromising their welfare and potentially reducing replicability between studies. The use of non-aversive handling (e.g., tunnel handling or...

This is the 5th volume of selected discussions that took place on the electronic Laboratory Animal Refinement & Enrichment Forum between February 2016 and December 2019. The forum was created in October 2002; it allows...

Rabbits are popular companion animals. There are numerous welfare issues affecting the majority of the population, including the fact that most rabbits are fearful when handled. Pet Remedy ™ (Unex Designs) is an herbal product...

Millions of mice are used every year for scientific research, representing the majority of scientific procedures conducted on animals. The standard method used to pick up laboratory mice for general husbandry and experimental procedures is...

The Named Veterinary Surgeon (NVS) at the Royal Veterinary College in London suggested providing post-operative pain relief (carprofen) to rats in raspberry flavoured jelly to avoid the use of s.c. injections. This article provides a...

Zoos use ambassador animals in educational programs featuring close contact with humans. Chinchillas (Chinchilla lanigera) at the Saint Louis Zoo are retrieved for programs by a keeper wearing brown handling gloves, but green cleaning gloves...

Skin closure clips are widely used within the University of Edinburgh to close incision sites for various procedures in both rats and mice. In rats the skin closure clips very often come out as a...

Mice are social animals hence group-housing of mice is preferred over individual housing. However, aggression in group-housed male mice under laboratory housing conditions is a well-known problem leading to serious health issues, including injury or...

In our facility, we use several hormone dependent tumour models with supplementation delivered via slow release subcutaneously implanted pellets, implanted via trochar. For example, breast tumours which rely on 17-β-Estradiol. Administration of E2 supplementation can...

There is a growing interest in the use of voluntarily displayed ongoing behaviours in laboratory animals to assess the pain experience. In rats, two behavioural pain scales, the Rat Grimace Scale (RGS, a facial expression...

Our rat training program teaches the standard method of handling and restraining that involves picking the rat up by the base of the tail and placing it on the wire mesh to be restrained. We...

As Animal Technologists we are constantly looking at refining and enriching the environmental conditions of all our animals to give them both physical exercise and mental stimulation regardless of their size. However, animals can still...

Handling can stimulate stress and anxiety in laboratory animals that negatively impacts welfare and introduces a confounding factor in many areas of research. Picking up mice by the tail is a major source of handling...

Mouse handling during cage changing and health evaluation has traditionally been performed by using forceps. This method was adopted as a biosecurity measure but can adversely affect employee ergonomics and rodent behavior. In this study...

Drug delivery in research on nonhuman animals in the laboratory is still challenging because it is usually invasive and stressful. Stress-free voluntary oral drug administration in water lacks precise control of dose and timing of...

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