Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 

This volume reviews the broad topic of welfare in nonhuman primates under human care. Chapters detail the history of primates in captivity, ethical and legal issues surrounding the use of nonhuman primates as entertainment or...

Shelters are stressful environments for domestic dogs (Canis familiaris). Evaluating dogs’ welfare is crucial to improve their life condition and to promote a better management of shelters. We aimed at verifying which variables improved welfare...

This study aimed to determine the effects of early-life physical and social enrichment on the ability of dairy heifers to integrate into a herd of mature cows. Fifty heifer calves were reared from the ages...

In captivity, intact male horses, due to their sexual drive, are usually socially isolated from other horses. This lifestyle strongly contrasts with that experienced by horses living in free-roaming, feral, or semi-feral conditions, where adult...

Halal and kosher slaughter have given the utmost importance to the sharpness of knives during the slaughter of animals. A sharp knife of appropriate dimension (blade length) makes slaughter less painful during neck severance and...

Animal stress is influenced by environmental factors, yet only a few studies have evaluated the effects of environmental stress on captive dogs. This study aimed to evaluate the effects of environmental and social enrichment on...

Social pair housing of calves has previously demonstrated positive impacts for calves, so this study aimed to compare the health and behaviour of calves kept in individual compared to pair housing on a single commercial...

To date, there is no official method for measuring horse welfare after transport. This study aimed to develop a scale to classify horses into four categories: good shape; light affected; affected; down (GLAD) based on...

In domestic conditions, adult stallions are mostly housed individually in internal stables to reduce the risk of injuries during social interactions. Social deprivation in horses results in physiological stress and behavioural problems. The aim of...

Domestic herbivores show a strong motivation to form associations with conspecifics and the social dynamics of any group is dependant on the individuals within the group. Thus, common farm management practices such mixing may cause...

Challenges and issues related to the use of pentobarbital euthanasia and disposal of animal remains within the US have recently been reviewed. Environmental and public health challenges increasingly necessitate consideration of alternative methods such as...

Current legislation in the United Kingdom stipulates that horses should not be slaughtered within sight of one another. However, abattoir personnel anecdotally report that, for semi-feral horses unused to restraint, co-slaughtering alongside a conspecific could...

Social housing for dairy calves has a range of benefits for social development, yet there is limited understanding of how social bonds form early in life. We characterized effects of early life social contact on...

Housing unweaned calves individually in barren environments negatively affects their growth, cognitive ability, and adaptability to environmental changes in later life. Social housing has been shown to improve those aspects, whereas physical environmental enrichment has...

The present study examined the effects of age at group housing and age at incremental hay supplementation in calf starters from 7.5 to 15% (dry matter, DM) and their interaction on growth performance, behavior, health...

Previously, dog personality traits that seem to correspond have been identified by the Dog Mentality Assessment (DMA) and the Behaviour and Personality Assessment in Dogs (BPH): Playfulness, Sociability, Curiosity/fearlessness (DMA) vs. Non-social fearfulness (BPH), Aggressiveness...

A fundamental understanding of behavior is essential to improving the welfare of billions of farm animals around the world. Despite living in an environment managed by humans, farm animals are still capable of making important...

Background: While most cattle and sheep in the United Kingdom are stunned before slaughter, non-stun methods are permitted to supply meat to specific consumers. This study aimed to identify the existing literature that compared animal...

Most dairy calves are housed individually in early ontogeny but social housing has positive effects on calf welfare including an advantage of social buffering, i.e., when negative effects of stress are mitigated through social support...

Despite an increase in awareness of their essential needs, many stallions continue to be kept in conditions limiting their social interactions and movement. To supplement the studies which highlight the effects of these practices on...

The National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) is working with industry to promote social housing during cardiovascular telemetry recordings within non-rodent safety pharmacology and toxicology studies. Following surveys...

The concept of the 3Rs (Refinement, Reduction and Replacement) has been used as a framework for improving the welfare of laboratory animals for the last half century. By establishing an animal-centric view on housing and...

Housing management of dairy calves is one of the factors that contributes to a successful rearing outcome. Individual housing of pre-weaned calves is thought to provide enhanced biosecurity and easier monitoring of the individual, and...

This literature review was aimed at analyzing whether stallion husbandry in groups is possible and desirable or poses risks. This was determined on the basis of different studies in order to be able to give...

Every animal species has particular environmental requirements that are essential for its welfare, and when these so-called “basic needs” are not fulfilled, the animals suffer. The basic needs of horses have been claimed to be...