Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every three months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Links to the full text for publications that appear in open access journals or are published on the AWI website are provided under the abstract.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic. Most publications older than 2010 can only be searched by keyword. 
In conclusion, the investigations carried out up to now demonstrate that during the early stages fish show high sensitivity to many types of stressors involving an array of responses to overcome alterations that could affect...
In dairy industry, quality of produced milk must be more important than quantity without a high somatic cells count (SCC) or pathogens causing mastitis of dairy cows and consumer diseases. Preserving the good health of...
Understanding the behavioral welfare of dogs in commercial breeding kennels (CBKs) is important for improving breeders’ management practices as well as dog welfare. In the current study, breeding dogs from CBKs were exposed to novel...
Weaning calves at a young age can generate great stress, but it is widely practiced in the industry despite that. So, to what extend weaning in early ages is more stressful than at later ones...
Appropriate end-points are integral to the refinement of laboratory animal experiments. Our recent experience has highlighted that ambiguity around end-points is hampering their adoption in experiments that cause severe suffering to fish. In toxicology, the...
There is an interest in alternative rearing systems that allow for prolonged cow-calf contact (CCC). Yet, a better understanding of cows’ affiliative behaviour in those systems is needed. We evaluated the effect of type of...
Maternal deprivation early in life has been shown to disrupt neonates’ development. Nevertheless, separating the young animals from their dams soon after birth remains a common practice in dairy farm husbandry. This study investigated the...
Objective, reliable behavioral tests are needed to refine on-site welfare assessments of dogs housed at commercial breeding (CB) kennels and provide a basis to inform predictions of their behavior when retired from such kennels. This...
The overpopulation of shelters and the increase of homeless dogs have become serious problems in many countries. One contributor to the number of both sheltered and homeless dogs is the abandonment and relinquishment of pet...
Early cow–calf separation management induced various welfare problems for dairy calves. We mimicked the maternal licking by manually brushing right after the Holstein female calves were born and during their first week of life, termed...
Optimising nest design for broiler breeders has benefits for both the animals and the producers. The welfare of the hens will increase by providing preferred housing, while also reducing eggs laid outside the nests. These...
Broiler breeders are feed-restricted during rearing to avoid the negative consequences of obesity during lay due to their genetic potential for fast growth rate. Feed restriction leads to chronic hunger, frustration and lack of satiety...
Weaning from milk is stressful. We aimed to investigate the effect of three weaning strategies on feed intake and behavior of dairy goat kids. Forty-five, 3-month-old Saanen cross goats were allocated to one of three...
Plasma concentrations of progesterone (P4) and 17β‐oestradiol (E2) in juvenile, pre‐ovulatory, early, mid‐ or late pregnancy stages of female blue sharks Prionace glauca were analysed. Concentrations of P4 were significantly higher in pregnant than in...
This article describes the successful rehoming of 60 Malawi cichlids from a vivarium at Stanford University to San Diego Zoo and the California Academy of Sciences.
Endangered whooping cranes (Grus americana ) have been produced in captivity for reintroduction programs since the 1980s, using techniques such as artificial insemination, multiple clutching, and captive‐rearing to speed recovery efforts. Chicks are often hand‐reared...
In dogs, the social and spatial restriction associated with living in a kennel environment could lead to chronic stress and the development of abnormal behaviors (“kennel-dog syndrome”). However, little is known about how kenneled dogs...
Chickens cannot independently thermoregulate at hatch and lack opportunity to behaviorally thermoregulate with a hen in the egg layer industry, thus barns are heated to thermoneutral temperatures. Dark brooders are low-energy-consuming hot plates, which may...
This is the 5th volume of selected discussions that took place on the electronic Laboratory Animal Refinement & Enrichment Forum between February 2016 and December 2019. The forum was created in October 2002; it allows...
Here we describe the behaviour of a female blonde capuchin (Sapajus flavius) towards her dead infant and discuss possible explanations linked to the anecdotal event. We conducted our study in a fragment of Atlantic forest...
Female lambs have a stronger attachment with their mothers and show a greater susceptibility to stressors than male lambs. Male lambs grow faster than female lambs do, achieving a greater nutritional independence from their mothers...
Broiler breeders are kept for the production of fertile eggs. They face serious welfare problems like aggressive behaviour of the males towards females during mating. Furthermore, broiler breeders are usually kept without perches which are...
Unwanted dogs are an international problem, and rehoming organisations are tasked with finding many of them appropriate homes. Whilst the processes involved in assessing dogs' suitability for rehoming have received considerable academic attention, the policies...
The most widespread reproductive rhythm practiced in rabbit farming is based on artificial insemination (AI) performed at around 11 days pospartum. This approach results in high production rates, but requires high yearly replacement of animals...
Isolation during calving is a common dairy cow behavior, however it has not been examined in large outdoor group settings. The provision of “hides” was monitored for its impact on calving location and cow–calf behavior...

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